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adsorption spectra, and they exhibit different deformation and immobilization features under photoirradiation. Therefore, information can be obtained about the photoimmobilization mechanisms by comparing these two types of azopolymers. First, the authors examined the relationship between immobilization ef ciency and the indented depth with respect to the photoirradiation time and the speci c azobenzene moiety. Second, they compared the relationship between immobilization ef ciency and chemical structure, and elucidated how this correlated with the photoisomerization properties and the retention rate of immobilized antibodies. The photodeformation capabilities of the CN-azopolymer and the H-azopolymer were examined by determining the depth of the indents formed by polystyrene microspheres under LED irradiation. After photoirradiation and removal of the microspheres, regularly arranged indented patterns formed by the microspheres were observed on the surfaces of the azopolymers. The depths of the indents were plotted as a function of irradiation time for several kinds of azopolymers, as shown in Fig. 9.14. The indent depths in the azopolymer increased with increasing irradiation time. The depths of the indents saturated and reached a maximum after 30 min of photoirradiation for each of the azopolymers. The saturated depths were lowest in those azopolymers with the lowest content of azobenzene moieties. These results indicate that the photoresponsive moiety plays an important role in inducing photodeformation and that the indent depth is related to the content of the azobenzene in the azopolymers. There were no differences in photodeformation capabilities between the CN- and

30 45 Irradiation time (min)

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Figure 9.14. Changes in the depth of the dent as a function of photoirradiation time for CN-azopolymers (solid gures) and H-azopolymers (open gures). The numbers show the weight content of the azo moieties. The open crosses show the control experiment using PMMA. Source: Narita, 2007. Reprinted with permission.

That CSS code would put the sticky post in a light grey box with a slightly darker frame, and 18pixel-sized default font size. Here it is in action, within a basic loop:

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H-azopolymers, even though they contained different types of azobenzene. These results show that H-azopolymers could exhibit immobilization capabilities similar to CN-azopolymers, despite the differences in their chemical structures. Photoimmobilization of IgGs was achieved by performing photoirradiation for 30 min to examine the ef ciency of the immobilization process. The relative ef ciencies of the photoimmobilization processes on the different azopolymers were plotted as a function of their azobenzene contents, as shown in Fig. 9.15. The immobilization ef ciencies of both the CN- and H-azopolymers. increased with azobenzene content up to B30 wt% and then became saturated, although the saturated values were different between the CN- and H-azopolymers. This result indicates that an azobenzene moiety that exhibits photoisomerization is essential to immobilize IgGs on azopolymers when using a photoimmobilization process. Next, the authors con rmed the relationship between the immobilization of IgG and the deformation ef ciency of the azopolymer. The relative immobilization ef ciencies were plotted as a function of indent depth, as shown in Fig. 9.16. Incremental changes in the immobilization ef ciency were observed by increasing the depth of the indents. However, the immobilization ef ciency of the Hazopolymers was higher than that of the CN-azopolymers across the whole range, and this is also shown in Fig. 9.15. This difference shows that the degree of photoimmobilization is not only affected by the deformation capability but is also a property of the surface of the azopolymer and is related to the chemical structure of the azobenzene that is incorporated in the azopolymer. Whitesides and coworkers have also reported that immobilization is in uenced by the properties of the surface (Ostuni et al., 2001).

Relative efficiency of immobilization (%)

Web strategist Jeremiah Owyang (www.web-strategist.com) explains how measurement has become or should be a priority for online communities in a comment posted on February 25, 2009 (www.web-strategist.com/blog/ 2009/01/28/community-managers-must-deliver-roi-tips-forsurviving-a-recession). Community managers must educate stakeholders and management, Jeremiah says. Community managers have to be in the thick of it and stay connected, but they also have to be able to quickly communicate changes back to internal company stakeholders. That means metrics. Forum One Communications oated their Online Community Metrics survey (www.onlinecommunityreport. com/uploads/metrics.pdf) in February of 2007. Fifty came back out of 150, an applause-worthy response rate in anybody s book. They asked a number of questions of online community professionals, including Which community metrics do you track Among the top metrics, the most important were: Unique Visitors New Member Registrations Page Views Retention/Attrition Member Loyalty Member Satisfaction Most Active Members

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:Azo(CN)polymer :Azo(H)polymer :Standard polymer(CN39)

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0 0 20 40 60 Azobenzene content (wt%) 80 100

Figure 9.15. Dependence of the relative immobilization ef ciency of Cy-5labeled antibodies on azopolymer content. The solid diamonds and open circles represent CN- and H-azopolymers, respectively. CN-39 was used as the standard polymer, as shown by the solid diamond in an open diamond. Source: Narita, 2007. Reprinted with permission.

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